Modeling the Impact of Amino Acids and Copper on Monoclonal Antibody Productiveness and Glycosylation: A Modular Strategy
- In manufacturing monoclonal antibodies (mAbs), it’s essential to have the ability to predict how course of situations and dietary supplements have an effect on antibody productiveness and high quality attributes, particularly glycosylation. Supplemental inputs, corresponding to amino acids and hint metals within the media, have been reported to have an effect on cell metabolism and glycosylation; understanding and quantifying their results are important for efficient course of improvement.
- Our goal is to current and validate, by way of a commercially related cell tradition course of, a method for capturing such results quantitatively and effectively in a mannequin. Whereas at present out there fashions can predict mAb manufacturing or glycosylation dynamics precisely beneath particular course of configurations, adapting them to new processes stays difficult, as a result of it includes modifying all the mannequin construction and sometimes requires some mechanistic understanding of how cells behave beneath new situations.
- Right here, we current a modular modeling method for adapting an current mannequin for a fed-batch Chinese language hamster ovary (CHO) cell tradition course of with out structural modifications or mechanistic perception. As an alternative, we use information, obtained from designed experimental perturbations in media concentrations of asparagine, glutamate, and copper, to coach and validate a supplemental enter impact mannequin, which approximates residual course of dynamics when supplemental inputs deviate from the baseline, and which is used to “patch” the prevailing mannequin.
- The mixed mannequin can be utilized for process-specific, model-based course of improvement to enhance productiveness and to fulfill product high quality targets extra effectively. Our mannequin was developed for a selected CHO cell line; nonetheless, the modeling methodology and evaluation are usually relevant to different cell traces and cell varieties. This text is protected by copyright. All rights reserved.
Alpha-bungarotoxin CF488a |
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| 9-00005 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF568 |
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| 9-00006 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF568 |
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| 9-00006 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF594 |
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| 9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin CF594 |
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| 9-00007 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF633 |
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| 9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-bungarotoxin, CF633 |
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| 9-00009 | Biotium |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin |
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| 00010-1 | Biotium | 1MG | EUR 201.6 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Fluorescein-Alpha-bungarotoxin |
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| 00011 | Biotium | 500uG | EUR 440.4 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Tetramethylrhodamine-Alpha-bungarotoxin |
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| 00012 | Biotium | 500uG | EUR 459.6 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Fluorescein-alpha-bungarotoxin: (10x50ug) |
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| 00013 | Biotium | 10ST | EUR 500.4 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug) |
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| 00014 | Biotium | 10ST | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug) |
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| 00015 | Biotium | 500uG | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug) |
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| 00016 | Biotium | 10ST | EUR 627.6 |
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Description: Minimum order quantity: 1 unit of 10ST |
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Biotin-xx-a-bungarotoxin |
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| 00017 | Biotium | 500uG | EUR 519.6 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-Bungarotoxin, CF555 conjugate |
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| 9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-Bungarotoxin, CF555 conjugate |
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| 9-00018 | Biotium |
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Description: Minimum order quantity: 1 unit of 100ug |
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Biotin-cAMP, diisopropylethylammonium salt: |
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| 00020 | Biotium | 1MG | EUR 376.8 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Biotin-cAMP, diisopropylethylammonium salt: (20x50ug) |
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| 00020-1 | Biotium | 20ST | EUR 501.6 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Biotin-cGMP, diisopropylethylammonium salt: |
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| 00021 | Biotium | 1MG | EUR 412.8 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Biotin-cGMP, diisopropylethylammonium salt: (20x50ug) |
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| 00021-1 | Biotium | 20ST | EUR 537.6 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Cyanine 644-cAMP: |
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| 00022 | Biotium | 1MG | EUR 589.2 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Cyanine 644-cAMP: (20x50ug) |
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| 00022-1 | Biotium | 20ST | EUR 750 |
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Description: Minimum order quantity: 1 unit of 20ST |
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Fluorescein Methotrexate, triammonium salt: |
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| 00023 | Biotium | 1MG | EUR 337.2 |
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Description: Minimum order quantity: 1 unit of 1MG |
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Staurosporine |
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| 00025 | Biotium | 100uG | EUR 166.8 |
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Description: Minimum order quantity: 1 unit of 100uG |
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Alpha-bungarotoxin, CF543 |
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| 00026 | Biotium | 500uG | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Rhodamine phalloidin 300u |
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| 00027 | Biotium | 300 | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300 |
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Biotin-xx-phalloidin |
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| 00028 | Biotium | 100U | EUR 513.6 |
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Description: Minimum order quantity: 1 unit of 100U |
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Fluorescein-phalloidin |
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| 00030 | Biotium | 300U | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300U |
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Rhodamine 110 phalloidin |
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| 00032 | Biotium | 300ST | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300ST |
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Sulforhodamine 101 (Texas-Red) Phalloidin |
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| 00033 | Biotium | 300EU | EUR 414 |
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Description: Minimum order quantity: 1 unit of 300EU |
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Phalloidin, CF405M |
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| 00034 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF405M |
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| 00034-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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CF488A-cAMP |
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| 00036 | Biotium | 100ug | EUR 206.4 |
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Description: Minimum order quantity: 1 unit of 100ug |
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CF640R-cAMP |
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| 00037 | Biotium | 100ug | EUR 206.4 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Phalloidin, CF555 |
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| 00040 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF555 |
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| 00040-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF647 |
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| 00041 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF647 |
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| 00041-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF488A |
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| 00042 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF488A |
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| 00042-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF543 |
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| 00043 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF543 |
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| 00043-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF568 |
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| 00044 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF568 |
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| 00044-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF594 |
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| 00045 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF594 |
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| 00045-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF633 |
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| 00046 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF633 |
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| 00046-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF660R |
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| 00047 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF660R |
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| 00047-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF680R |
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| 00048 | Biotium | 300U | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF680R |
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| 00048-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF350 |
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| 00049 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF350 |
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| 00049-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF640R |
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| 00050 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF640R |
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| 00050-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF532 |
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| 00051 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF532 |
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| 00051-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF660C |
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| 00052 | Biotium | 300U | EUR 530.4 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF660C |
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| 00052-T | Biotium | 50U | EUR 159.6 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF680 |
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| 00053 | Biotium | 300U | EUR 559.2 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF680 |
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| 00053-T | Biotium | 50U | EUR 176.4 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF430 |
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| 00054 | Biotium | 300U | EUR 538.8 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF430 |
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| 00054-T | Biotium | 50U | EUR 166.8 |
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Description: Minimum order quantity: 1 unit of 50U |
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Phalloidin, CF440 |
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| 00055 | Biotium | 300U | EUR 538.8 |
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Description: Minimum order quantity: 1 unit of 300U |
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Phalloidin, CF440 |
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| 00055-T | Biotium | 50U | EUR 166.8 |
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Description: Minimum order quantity: 1 unit of 50U |
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Cholera Toxin Subunit B, CF488A conjugate |
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| 00070 | Biotium | 100ug | EUR 313.2 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF568 conjugate |
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| 00071 | Biotium | 100ug | EUR 313.2 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF594 conjugate |
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| 00072 | Biotium | 100ug | EUR 313.2 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF640R conjugate |
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| 00073 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF532 conjugate |
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| 00074 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF543 conjugate |
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| 00075 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF620R conjugate |
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| 00076 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF633 conjugate |
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| 00077 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF660R conjugate |
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| 00078 | Biotium | 100ug | EUR 316.8 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Cholera Toxin Subunit B, CF680R conjugate |
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| 00079 | Biotium | 100ug | EUR 338.4 |
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Description: Minimum order quantity: 1 unit of 100ug |
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Human Transferrin, CF488A conjugate |
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| 00081 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF543 conjugate |
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| 00082 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF568 conjugate |
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| 00083 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF594 conjugate |
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| 00084 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF640R conjugate |
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| 00085 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF680R conjugate |
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| 00086 | Biotium | 1mg | EUR 178.8 |
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Description: Minimum order quantity: 1 unit of 1mg |
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Human Transferrin, CF750 conjugate |
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| 00087 | Biotium | 1mg | EUR 186 |
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Description: Minimum order quantity: 1 unit of 1mg |
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HIV-1 tat recombinant antigen |
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| 00110-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HIV-1 tat recombinant antigen full length 15 kDa |
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HIV-1 tat recombinant antigen |
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| 00110-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: HIV-1 tat recombinant antigen full length 15 kDa |
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HIV-1 GAG P24 Recombinant Antigen |
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| 00111-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa |
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HIV-1 GAG P24 Recombinant Antigen |
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| 00111-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa |
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HIV-1 nef recombinant antigen |
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| 00112-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa |
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HIV-1 nef recombinant antigen |
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| 00112-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa |
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HIV-1 env gp41 recombinant antigen |
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| 00113-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa |
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HIV-1 env gp41 recombinant antigen |
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| 00113-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa |
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HIV-2 GP 36 Recombinant Antigen |
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| 00114-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa |
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HIV-2 GP 36 Recombinant Antigen |
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| 00114-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa |
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HCV core recombinant antigen |
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| 00115-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa |
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HCV core recombinant antigen |
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| 00115-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa |
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HCV core recombinant antigen, Biotin conjugate |
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| 00115-V-B-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate. |
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HCV core recombinant antigen, Biotin conjugate |
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| 00115-V-B-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate. |
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HCV core recombinant antigen, FITC conjugate |
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| 00115-V-F-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate. |
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HCV core recombinant antigen, FITC conjugate |
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| 00115-V-F-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate. |
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HCV core recombinant antigen, Rhodamine |
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| 00115-V-R-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate. |
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HCV core recombinant antigen, Rhodamine |
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| 00115-V-R-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate. |
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HCV NS4 recombinant antigen NS4a+b |
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| 00116-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. |
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HCV NS4 recombinant antigen NS4a+b |
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| 00116-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. |
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HCV NS4 recombinant antigen NS4a+b, Biotin |
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| 00116-V-B-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate. |
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HCV NS4 recombinant antigen NS4a+b, Biotin |
|||
| 00116-V-B-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate. |
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HCV NS4 recombinant antigen NS4a+b, FITC |
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| 00116-V-F-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate. |
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HCV NS4 recombinant antigen NS4a+b, FITC |
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| 00116-V-F-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate. |
|||
HCV NS4 recombinant antigen NS4a+b, Rhodamine |
|||
| 00116-V-R-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate. |
|||
HCV NS4 recombinant antigen NS4a+b, Rhodamine |
|||
| 00116-V-R-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate. |
|||
HCV NS3 recombinant antigen |
|||
| 00117-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa |
|||
HCV NS3 recombinant antigen |
|||
| 00117-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa |
|||
HBV core recombinant antigen |
|||
| 00120-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa |
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HBV core recombinant antigen |
|||
| 00120-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa |
|||
HBV core recombinant antigen, Delta |
|||
| 00121-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa |
|||
HBV core recombinant antigen, Delta |
|||
| 00121-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa |
|||
HBV surface recombinant antigen HBsAg |
|||
| 00122-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant |
|||
HBV surface recombinant antigen HBsAg |
|||
| 00122-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant |
|||
HBV surface recombinant antigen HBsAg antigen |
|||
| 00123-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity |
|||
HBV surface recombinant antigen HBsAg antigen |
|||
| 00123-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity |
|||
HBV surface recombinant antigen HBsAg antigen |
|||
| 00124-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant |
|||
HBV surface recombinant antigen HBsAg antigen |
|||
| 00124-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant |
|||
HEV (Birma) ORF2 recombinant antigen |
|||
| 00131-V-01mg | Virogen | 0,1 mg | EUR 321 |
|
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659. |
|||
HEV (Birma) ORF2 recombinant antigen |
|||
| 00131-V-1000ug | Virogen | 1000 ug | EUR 1539 |
|
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659. |
|||
HEV (Birma) ORF2 recombinant antigen |
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| 00132-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461. |
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HEV (Birma) ORF2 recombinant antigen |
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| 00132-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461. |
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AA 403-461 HEV Birma ORF2 Recombinant Antigen |
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| 00133-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123. |
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AA 403-461 HEV Birma ORF2 Recombinant Antigen |
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| 00133-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123. |
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T, Pallidum recombinant antigen p15 |
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| 00141-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: T. Pallidum recombinant antigen p15 (full length) |
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T, Pallidum recombinant antigen p15 |
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| 00141-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: T. Pallidum recombinant antigen p15 (full length) |
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T, Pallidum recombinant antigen p17 |
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| 00142-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: T. Pallidum recombinant antigen p17 (full length) |
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T, Pallidum recombinant antigen p17 |
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| 00142-V-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: T. Pallidum recombinant antigen p17 (full length) |
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T, Pallidum recombinant antigen p45 |
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| 00143-V-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: T. Pallidum recombinant antigen p45 (full length) |
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Biased anti-idiotype response in rabbits results in high-affinity monoclonal antibodies to biologics
Antibody formation to human(ized) therapeutic antibodies in people is extremely skewed towards anti-idiotype responses, in all probability as a result of the idiotype is the one ‘international’ a part of the antibody molecule. Right here, we analyzed antibody responses to F(ab’)2 fragments of a panel of 17 human(ized) therapeutic antibodies in rabbits. Homology between the rabbit germline and the human(ized) antibodies is average not just for the variable domains (each the complementarity-determining regions and the framework areas), but in addition for the fixed domains (66% or much less).
However, we noticed a extremely skewed anti-idiotype response in all circumstances, with as much as >90% of the antibodies directed towards the idiotype. These outcomes point out that the idiotype could also be inherently immunodominant. We used these biased responses to lift monoclonal rabbit anti-idiotype antibodies in opposition to secukinumab, ustekinumab, reslizumab, mepolizumab, palivizumab, and dupilumab and display the potential to develop delicate pharmacokinetic assays with these antibodies.
Choice and Characterization of YKL-40-Concentrating on Monoclonal Antibodies from Human Artificial Fab Phage Show Libraries
YKL-40, often known as chitinase-3-like 1 (CHI3L1), is a glycoprotein that’s expressed and secreted by varied cell varieties, together with cancers and macrophages. Resulting from its implications for and upregulation in quite a lot of illnesses, together with inflammatory situations, fibrotic issues, and tumor progress, YKL-40 has been thought of as a big therapeutic biomarker. Right here, we used a phage show to develop novel monoclonal antibodies (mAbs) focusing on human YKL-40 (hYKL-40).
Human artificial antibody phage show libraries had been panned in opposition to a recombinant hYKL-40 protein, yielding seven distinctive Fabs (Antigen-binding fragment), of which two Fabs (H1 and H2) had been non-aggregating and thermally steady (75.5 °C and 76.5 °C, respectively) and had excessive obvious affinities (OkD = 2.Three nM and 4.Zero nM, respectively).
Reformatting the Fabs into IgGs (Immunoglobulin Gs) elevated their obvious affinities (notably, for H1 and H2, OkD = 0.5 nM and 0.Three nM, respectively), presumably because of the results of avidity, with little change to their non-aggregation property. The six anti-hYKL-40 IgGs had been analyzed utilizing a trans-well migration assay in vitro, revealing that three clones (H1, H2, and H4) had been notably efficient in lowering cell migration from each A549 and H460 lung most cancers cell traces.
The three clones had been additional analyzed in an in vivo animal check that assessed their anti-cancer actions, demonstrating that the tumor space and the variety of tumor nodules had been considerably decreased within the lung tissues handled with H1 (IgG). Given its excessive affinity and fascinating properties, we count on that the H1 anti-hYKL-40 mAb can be an appropriate candidate for growing anti-cancer therapeutics.
Polyclonal BRD2 polyclonal antibody |
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| APR00374G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal JMJD2c polyclonal antibody |
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| APR00375G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JMJD2c polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BMI1 polyclonal antibody |
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| APR00376G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SHH Polyclonal Antibody |
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| APR00434G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SHH Polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal ETO polyclonal antibody |
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| APR00435G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ETO polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EZH2 polyclonal antibody |
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| APR00436G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal RARA polyclonal antibody |
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| APR00437G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RARA polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MYH11 polyclonal antibody |
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| APR00438G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYH11 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EZH2 polyclonal antibody |
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| APR00439G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal p53K372me1 polyclonal antibody |
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| APR00440G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human p53K372me1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CIITA polyclonal antibody |
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| APR00442G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CIITA polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal p53 polyclonal antibody |
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| APR00443G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human p53 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CHD4 polyclonal antibody |
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| APR00444G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal HDAC1 polyclonal antibody |
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| APR00445G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Spt16 polyclonal antibody |
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| APR00446G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BRD2 polyclonal antibody |
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| APR00447G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal G9a polyclonal antibody |
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| APR00448G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human G9a polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Hira polyclonal antibody |
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| APR00449G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Hira polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal JMJD2c polyclonal antibody |
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| APR00450G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JMJD2c polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SPI1 polyclonal antibody |
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| APR00451G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SPI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BCL7C polyclonal antibody |
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| APR00452G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BCL7C polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal LRWD1 polyclonal antibody |
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| APR00455G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LRWD1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal LYDG10 polyclonal antibody |
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| APR00456G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LYDG10 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BMI1 polyclonal antibody |
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| APR00457G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal KDM4D polyclonal antibody |
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| APR00458G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KDM4D polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CHD4 polyclonal antibody |
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| APR00459G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Spt16 polyclonal antibody |
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| APR00460G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal FKBP51 polyclonal antibody |
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| APR15992G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKBP51 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal JARID2 polyclonal antibody |
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| APR12450G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JARID2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Med26 polyclonal antibody |
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| APR12517G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Med26 polyclonal antibody |
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| APR12518G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| APR12525G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| APR12526G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| APR12527G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SOX4 polyclonal antibody |
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| APR13475G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SOX4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal YY1 polyclonal antibody |
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| APR14018G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human YY1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PADI4 polyclonal antibody |
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| APR08916G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PADI4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Rtf1 polyclonal antibody |
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| APR11187G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Rtf1 polyclonal antibody |
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| APR11188G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PIWIL2 polyclonal antibody |
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| APR17846G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PIWIL2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal AKR1B10 Polyclonal Antibody |
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| APG01659G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AKR1B10 Polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal ZMYND8 polyclonal antibody |
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| AMM08559G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZMYND8 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PML polyclonal antibody |
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| AMR09397G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PML polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PPARG polyclonal antibody |
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| AMR09459G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PPARG polyclonal antibody |
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| AMR09486G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal AntibodyP1 Polyclonal Antibody |
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| ES11752-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against P1. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP1 Polyclonal Antibody |
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| ES11752-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against P1. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP2 Polyclonal Antibody |
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| ES10016-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against P2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP2 Polyclonal Antibody |
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| ES10016-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against P2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP4 Polyclonal Antibody |
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| ES10017-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against P4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP4 Polyclonal Antibody |
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| ES10017-50ul | ELK Biotech | 50ul | EUR 248.4 |
|
Description: A Rabbit Polyclonal antibody against P4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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Polyclonal AntibodyP5 Polyclonal Antibody |
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| ES4829-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against P5 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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Polyclonal AntibodyP5 Polyclonal Antibody |
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| ES4829-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against P5 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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Polyclonal AntibodyP3 Polyclonal Antibody |
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| ES6454-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against P3 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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Polyclonal AntibodyP3 Polyclonal Antibody |
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| ES6454-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against P3 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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Polyclonal sRANK Ligand Polyclonal Antibody |
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| APR00294G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human sRANK Ligand Polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal FGF-1 Polyclonal Antibody |
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| APR00423G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FGF-1 Polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal RFX-AP polyclonal antibody |
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| APR00441G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RFX-AP polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EIF2C1/2 polyclonal antibody |
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| APR00453G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EIF2C1/2 polyclonal . This antibody is tested and proven to work in the following applications: |
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