Modeling the Impact of Amino Acids and Copper on Monoclonal Antibody Productiveness and Glycosylation: A Modular Strategy
- In manufacturing monoclonal antibodies (mAbs), it’s essential to have the ability to predict how course of situations and dietary supplements have an effect on antibody productiveness and high quality attributes, particularly glycosylation. Supplemental inputs, corresponding to amino acids and hint metals within the media, have been reported to have an effect on cell metabolism and glycosylation; understanding and quantifying their results are important for efficient course of improvement.
- Our goal is to current and validate, by way of a commercially related cell tradition course of, a method for capturing such results quantitatively and effectively in a mannequin. Whereas at present out there fashions can predict mAb manufacturing or glycosylation dynamics precisely beneath particular course of configurations, adapting them to new processes stays difficult, as a result of it includes modifying all the mannequin construction and sometimes requires some mechanistic understanding of how cells behave beneath new situations.
- Right here, we current a modular modeling method for adapting an current mannequin for a fed-batch Chinese language hamster ovary (CHO) cell tradition course of with out structural modifications or mechanistic perception. As an alternative, we use information, obtained from designed experimental perturbations in media concentrations of asparagine, glutamate, and copper, to coach and validate a supplemental enter impact mannequin, which approximates residual course of dynamics when supplemental inputs deviate from the baseline, and which is used to “patch” the prevailing mannequin.
- The mixed mannequin can be utilized for process-specific, model-based course of improvement to enhance productiveness and to fulfill product high quality targets extra effectively. Our mannequin was developed for a selected CHO cell line; nonetheless, the modeling methodology and evaluation are usually relevant to different cell traces and cell varieties. This text is protected by copyright. All rights reserved.
variable volume 2ml -10ml |
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| -LHP1-V2000 | Phoenix instrument | each | EUR 109 |
variable volume 5 -50 µl |
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| -LHP1-V5 | Phoenix instrument | each | EUR 109 |
variable volume 50 -200 µl |
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| -LHP1-V50 | Phoenix instrument | each | EUR 109 |
fix volume 10 µl, autoclavable |
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| -LHP2-F10 | Phoenix instrument | each | EUR 89 |
fix volume 100 µl, autoclavable |
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| -LHP2-F100 | Phoenix instrument | each | EUR 89 |
fix volume 1000 µl, autoclavable |
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| -LHP2-F1000 | Phoenix instrument | each | EUR 89 |
fix volume 20 µl, autoclavable |
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| -LHP2-F20 | Phoenix instrument | each | EUR 89 |
fix volume 200 µl, autoclavable |
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| -LHP2-F200 | Phoenix instrument | each | EUR 89 |
fix volume 2000 µl, autoclavable |
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| -LHP2-F2000 | Phoenix instrument | each | EUR 89 |
fix volume 25 µl, autoclavable |
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| -LHP2-F25 | Phoenix instrument | each | EUR 89 |
fix volume 250 µl, autoclavable |
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| -LHP2-F250 | Phoenix instrument | each | EUR 89 |
fix volume 5 µl, autoclavable |
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| -LHP2-F5 | Phoenix instrument | each | EUR 89 |
fix volume 50 µl, autoclavable |
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| -LHP2-F50 | Phoenix instrument | each | EUR 89 |
fix volume 500 µl, autoclavable |
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| -LHP2-F500 | Phoenix instrument | each | EUR 89 |
fix volume 5000 µl, autoclavable |
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| -LHP2-F5000 | Phoenix instrument | each | EUR 89 |
variable volume 0,1 -2,5 µl, autoclavable |
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| -LHP2-V01 | Phoenix instrument | each | EUR 139 |
variable volume 0,5 -10 µl, autoclavable |
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| -LHP2-V05 | Phoenix instrument | each | EUR 139 |
variable volume 10 -100 µl, autoclavable |
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| -LHP2-V10 | Phoenix instrument | each | EUR 139 |
variable volume 100-1000 µl, autoclavble |
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| -LHP2-V100 | Phoenix instrument | each | EUR 139 |
variable volume 1000 -5000 µl, autoclavable |
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| -LHP2-V1000 | Phoenix instrument | each | EUR 139 |
variable volume 2 -20 µl, autoclavable |
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| -LHP2-V2 | Phoenix instrument | each | EUR 139 |
variable volume 20 -200 µl, autoclavable |
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| -LHP2-V20 | Phoenix instrument | each | EUR 139 |
variable volume 200 -1000 µl, autoclavable |
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| -LHP2-V200 | Phoenix instrument | each | EUR 139 |
variable volume 5 -50 µl, autoclavable |
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| -LHP2-V5 | Phoenix instrument | each | EUR 139 |
variable volume 50 -200 µl, autoclavable |
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| -LHP2-V50 | Phoenix instrument | each | EUR 139 |
variable volume 0,1 -2,5 µl, autoclavable |
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| -LHP3-V01 | Phoenix instrument | each | EUR 169 |
variable volume 0,5 -10 µl, autoclavable |
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| -LHP3-V05 | Phoenix instrument | each | EUR 169 |
variable volume 10 -100 µl, autoclavable |
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| -LHP3-V10 | Phoenix instrument | each | EUR 169 |
variable volume 100-1000 µl, autoclavable |
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| -LHP3-V100 | Phoenix instrument | each | EUR 169 |
variable volume 2 -20 µl, autoclavable |
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| -LHP3-V2 | Phoenix instrument | each | EUR 169 |
variable volume 20 -200 µl, autoclavable |
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| -LHP3-V20 | Phoenix instrument | each | EUR 169 |
variable volume 30 -300 µl, autoclavable |
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| -LHP3-V30 | Phoenix instrument | each | EUR 169 |
variable volume 5 -50 µl, autoclavable |
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| -LHP3-V5 | Phoenix instrument | each | EUR 169 |
10 mm Diameter Solid Titanium Tip |
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| 0-120-0009 | Biologics | 10 mm | EUR 504 |
13 mm Diameter Tapped Titanium Tip |
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| 0-120-0010 | Biologics | 13 mm | EUR 595 |
13 mm Diameter Solid Titanium Tip |
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| 0-120-0011 | Biologics | 13 mm | EUR 533 |
19 mm Diameter Tapped Titanium Tip |
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| 0-120-0012 | Biologics | 19 mm | EUR 639 |
19 mm Diameter Solid Titanium Tip |
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| 0-120-0013 | Biologics | 19 mm | EUR 570 |
25 mm Diameter Tapped Titanium Tip |
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| 0-120-0014 | Biologics | 25 mm | EUR 682 |
25 mm Diameter Solid Titanium Tip |
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| 0-120-0015 | Biologics | 25 mm | EUR 614 |
Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip |
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| 0-120-0016 | Biologics | 13 mm | EUR 86 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
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Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip |
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| 0-120-0017 | Biologics | 19 mm | EUR 92 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
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Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip |
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| 0-120-0018 | Biologics | 25 mm | EUR 100 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
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Titanium Cup Tip, 250 ml |
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| 0-120-0019 | Biologics | 250 ml | EUR 1377 |
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Description: includes Interface Washers |
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Microtube Tray, 8 Position (for 250 ml Cup Tip) |
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| 0-120-0021 | Biologics | each | EUR 113 |
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Description: includes Interface Washers |
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Continuous Flow Chamber |
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| 0-120-0026 | Biologics | each | EUR 774 |
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Description: includes Interface Washers |
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Interface Washers 1.5” Diameter, 5/Pkg |
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| 0-120-003 | Biologics | 1.5'' Diameter | EUR 31 |
13 mm Diameter Solid Titanium Extender Tip |
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| 0-120-0032 | Biologics | 13 mm | EUR 301 |
13 mm Diameter Tapped Titanium Extender Tip |
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| 0-120-0033 | Biologics | 13 mm | EUR 362 |
19 mm Diameter Solid Titanium Extender Tip |
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| 0-120-0034 | Biologics | 19 mm | EUR 315 |
19 mm Diameter Tapped Titanium Extender Tip |
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| 0-120-0035 | Biologics | 19 mm | EUR 375 |
25 mm Diameter Solid Titanium Extender Tip |
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| 0-120-0036 | Biologics | 25 mm | EUR 328 |
25 mm Diameter Tapped Titanium Extender Tip |
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| 0-120-0037 | Biologics | 25 mm | EUR 389 |
KoldPod, 1.5ml Micro Tube |
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| 0-120-0038 | Biologics | 1.5 ml | EUR 152 |
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Description: Thermo conductive tube pods |
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KoldPod, 15ml Conical Tube |
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| 0-120-0039 | Biologics | 15 ml | EUR 275 |
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Description: Thermo conductive tube pods |
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KoldPod, 50ml Conical Tube |
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| 0-120-0040 | Biologics | 50 ml | EUR 290 |
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Description: Thermo conductive tube pods |
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Model 150 V/T Ultrasonic Homogenizer |
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| 0-121-0002 | Biologics | 210-240V/50-60Hz | EUR 2920 |
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Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing. |
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Model 300 V/T Ultrasonic Homogenizer |
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| 0-122-0002 | Biologics | 210-240V/50-60Hz | EUR 3520 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample. |
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SONABOZ Sound Abating Chamber |
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| 0-125-0001 | Biologics | each | EUR 1020 |
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Description: Reduces cavitational sound emitted during processing. |
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Model 3000 Ultrasonic Homogenizer |
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| 0-127-0002 | Biologics | 210-240V/50-60Hz | EUR 4120 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample. |
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Model 3000MP Ultrasonic Homogenizer |
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| 0-128-0002 | Biologics | 210-240V/50-60Hz | EUR 4720 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing. |
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OMNICON® Zone Reader, 210-240V/50-60Hz |
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| 0-131-0002 | Biologics | 210-240V/50-60Hz | EUR 35200 |
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Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media. |
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OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz |
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| 0-134-0002 | Biologics | 210-240V/50-60Hz | EUR 32200 |
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Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample. |
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Peni Cylinder Dispenser with Manual Hopper |
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| 0-144-0002 | Biologics | each | EUR 5406 |
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Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish. |
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Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz |
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| 0-144-0003 | Biologics | 100-240V/50-60Hz | EUR 6254 |
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Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection. |
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Stainless Steel Peni Cylinder with Flat Face |
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| 0-144-0005 | Biologics | 6mm I.D. x 8mm O.D. x 10mm Long | EUR 399 |
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Description: sold in packages of 100 pieces |
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Stainless Steel Peni Cylinder with Chamfered Face |
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| 0-144-0006 | Biologics | 6mm I.D. x 8mm O.D. x 10mm Long | EUR 412 |
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Description: sold in packages of 100 pieces |
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Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA) |
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| 000-CUS | Alpha Diagnostics | Custom | EUR 602 |
Alpha-Bungarotoxin, CF®405S, 500 ug |
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| 00002 | Biotium | 1UG | EUR 527 |
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Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-bungarotoxin, CF405s |
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| 00002 | Cusabio | 500uG | EUR 594 |
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Description: Minimum order quantity: 1 unit of 500uG |
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Alpha-Bungarotoxin, CF®405S, 500 ug |
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| 00002-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®405S 100ug |
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| 00002-100ug | Biotium | 100uG | EUR 132 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®680R, 500 ug |
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| 9-00003 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®680R, 500 ug |
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| 00003-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®680R 100ug |
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| 9-00003 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®640R, 500 ug |
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| 9-00004 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®640R, 500 ug |
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| 00004-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®640R 100ug |
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| 9-00004 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®488A, 500 ug |
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| 9-00005 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®488A, 500 ug |
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| 00005-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin CF®488A 100ug |
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| 9-00005 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®568, 500 ug |
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| 9-00006 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®568, 500 ug |
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| 00006-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®568 100ug |
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| 9-00006 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®594, 500 ug |
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| 9-00007 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®594, 500 ug |
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| 00007-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin CF®594 100ug |
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| 9-00007 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®633, 500 ug |
|||
| 9-00009 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, CF®633, 500 ug |
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| 00009-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®633 100ug |
|||
| 9-00009 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only |
|||
| 0001-PP-001 | IBT Bioservices | 1 cell line (can order x amount) | EUR 12000 |
|
Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells |
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Alpha-Bungarotoxin, 1 mg |
|||
| 00010-1 | Biotium | 1MG | EUR 193 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Alpha-Bungarotoxin, 1 mg |
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| 00010-1-1 | Biotium | EA | EUR 193 |
Fluorescein-Alpha-Bungarotoxin, 500 ug |
|||
| 00011 | Biotium | 500uG | EUR 376 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Fluorescein-Alpha-Bungarotoxin, 500 ug |
|||
| 00011-1 | Biotium | EA | EUR 376 |
Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|||
| 00012 | Biotium | 500uG | EUR 394 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|||
| 00012-1 | Biotium | EA | EUR 394 |
Fluorescein-alpha-bungarotoxin, 10x50ug |
|||
| 00013 | Biotium | 10ST | EUR 436 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Fluorescein-alpha-bungarotoxin, 10x50ug |
|||
| 00013-1 | Biotium | EA | EUR 436 |
Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|||
| 00014 | Biotium | 10ST | EUR 494 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|||
| 00014-1 | Biotium | EA | EUR 494 |
Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|||
| 00015 | Biotium | 500uG | EUR 494 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|||
| 00015-1 | Biotium | EA | EUR 494 |
Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|||
| 00016 | Biotium | 10ST | EUR 560 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|||
| 00016-1 | Biotium | EA | EUR 560 |
Biotin-XX-A-Bungarotoxin, 500 ug |
|||
| 00017 | Biotium | 500uG | EUR 455 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
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Biotin-XX-A-Bungarotoxin, 500 ug |
|||
| 00017-1 | Biotium | EA | EUR 455 |
Alpha-Bungarotoxin, CF®555, 500 ug |
|||
| 9-00018 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®555, 500 ug |
|||
| 00018-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®555 100ug |
|||
| 9-00018 | Biotium |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Acrylamide, Chemzymes Ultra Pure® |
|||
| 00019-100 | Polysciences Europe GmbH | 100g | EUR 101.52 |
|
Description: 79-06-1 |
|||
Acrylamide, Chemzymes Ultra Pure® |
|||
| 00019-500 | Polysciences Europe GmbH | 500g | EUR 270 |
|
Description: 79-06-1 |
|||
Biotin-cAMP, 1 mg |
|||
| 00020 | Biotium | 1MG | EUR 298 |
|
Description: N/A |
|||
Biotin-cAMP, 1 mg |
|||
| 00020-1 | Biotium | 20ST | EUR 298 |
|
Description: N/A |
|||
Biotin-cAMP, 50 ug |
|||
| 00020-1-1 | Biotium | EA | EUR 414 |
Biotin-cGMP, 1 mg |
|||
| 00021 | Biotium | 1MG | EUR 331 |
|
Description: N/A |
|||
Biotin-cGMP, 1 mg |
|||
| 00021-1 | Biotium | 20ST | EUR 331 |
|
Description: N/A |
|||
Biotin-cGMP, 20x50 ug |
|||
| 00021-1-1 | Biotium | EA | EUR 447 |
Cyanine 644-cAMP, 1 mg |
|||
| 00022 | Biotium | 1MG | EUR 496 |
|
Description: N/A |
|||
Cyanine 644-cAMP, 1 mg |
|||
| 00022-1 | Biotium | 20ST | EUR 496 |
|
Description: N/A |
|||
Cyanine 644-cAMP, 20x50 ug |
|||
| 00022-1-1 | Biotium | EA | EUR 647 |
Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|||
| 00023 | Biotium | 1MG | EUR 285 |
|
Description: N/A |
|||
Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|||
| 00023-1 | Biotium | EA | EUR 285 |
Staurosporine |
|||
| 00025 | Biotium | 100uG | EUR 100 |
|
Description: N/A |
|||
Staurosporine |
|||
| 00025-1 | Biotium | EA | EUR 100 |
Alpha-Bungarotoxin, CF®543, 500 ug |
|||
| 00026 | Biotium | 500uG | EUR 527 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Alpha-Bungarotoxin, CF®543, 500 ug |
|||
| 00026-1 | Biotium | EA | EUR 527 |
Alpha-Bungarotoxin, CF®543, 100 ug |
|||
| 00026-100ug | Biotium | 1UG | EUR 132 |
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
|||
Rhodamine Phalloidin 300U |
|||
| 00027 | Biotium | 300 | EUR 364 |
|
Description: N/A |
|||
Rhodamine Phalloidin 300U |
|||
| 00027-1 | Biotium | EA | EUR 364 |
Biotin-XX-Phalloidin |
|||
| 00028 | Biotium | 100U | EUR 466 |
|
Description: N/A |
|||
Biotin-XX-Phalloidin |
|||
| 00028-1 | Biotium | EA | EUR 466 |
Fluorescein-Phalloidin |
|||
| 00030 | Biotium | 300U | EUR 364 |
|
Description: N/A |
|||
Biased anti-idiotype response in rabbits results in high-affinity monoclonal antibodies to biologics
Antibody formation to human(ized) therapeutic antibodies in people is extremely skewed towards anti-idiotype responses, in all probability as a result of the idiotype is the one ‘international’ a part of the antibody molecule. Right here, we analyzed antibody responses to F(ab’)2 fragments of a panel of 17 human(ized) therapeutic antibodies in rabbits. Homology between the rabbit germline and the human(ized) antibodies is average not just for the variable domains (each the complementarity-determining regions and the framework areas), but in addition for the fixed domains (66% or much less).
However, we noticed a extremely skewed anti-idiotype response in all circumstances, with as much as >90% of the antibodies directed towards the idiotype. These outcomes point out that the idiotype could also be inherently immunodominant. We used these biased responses to lift monoclonal rabbit anti-idiotype antibodies in opposition to secukinumab, ustekinumab, reslizumab, mepolizumab, palivizumab, and dupilumab and display the potential to develop delicate pharmacokinetic assays with these antibodies.
Choice and Characterization of YKL-40-Concentrating on Monoclonal Antibodies from Human Artificial Fab Phage Show Libraries
YKL-40, often known as chitinase-3-like 1 (CHI3L1), is a glycoprotein that’s expressed and secreted by varied cell varieties, together with cancers and macrophages. Resulting from its implications for and upregulation in quite a lot of illnesses, together with inflammatory situations, fibrotic issues, and tumor progress, YKL-40 has been thought of as a big therapeutic biomarker. Right here, we used a phage show to develop novel monoclonal antibodies (mAbs) focusing on human YKL-40 (hYKL-40).
Human artificial antibody phage show libraries had been panned in opposition to a recombinant hYKL-40 protein, yielding seven distinctive Fabs (Antigen-binding fragment), of which two Fabs (H1 and H2) had been non-aggregating and thermally steady (75.5 °C and 76.5 °C, respectively) and had excessive obvious affinities (OkD = 2.Three nM and 4.Zero nM, respectively).
Reformatting the Fabs into IgGs (Immunoglobulin Gs) elevated their obvious affinities (notably, for H1 and H2, OkD = 0.5 nM and 0.Three nM, respectively), presumably because of the results of avidity, with little change to their non-aggregation property. The six anti-hYKL-40 IgGs had been analyzed utilizing a trans-well migration assay in vitro, revealing that three clones (H1, H2, and H4) had been notably efficient in lowering cell migration from each A549 and H460 lung most cancers cell traces.
The three clones had been additional analyzed in an in vivo animal check that assessed their anti-cancer actions, demonstrating that the tumor space and the variety of tumor nodules had been considerably decreased within the lung tissues handled with H1 (IgG). Given its excessive affinity and fascinating properties, we count on that the H1 anti-hYKL-40 mAb can be an appropriate candidate for growing anti-cancer therapeutics.
Polyclonal AntibodyPC4 Polyclonal Antibody |
|||
| RD83593A-120uL | Reddot Biotech | 120μL | EUR 360 |
|
Description: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
|||
Polyclonal AntibodyPC4 Polyclonal Antibody |
|||
| RD83593A-200uL | Reddot Biotech | 200μL | EUR 630 |
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Description: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Polyclonal AntibodyPC4 Polyclonal Antibody |
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| RD83593A-60uL | Reddot Biotech | 60μL | EUR 204 |
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Description: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Polyclonal ETO polyclonal antibody |
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| APR00372G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ETO polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SHH Polyclonal Antibody |
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| APR00434G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SHH Polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal ETO polyclonal antibody |
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| APR00435G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ETO polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal p53 polyclonal antibody |
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| APR00443G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human p53 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal G9a polyclonal antibody |
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| APR00448G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human G9a polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal YY1 polyclonal antibody |
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| APR14018G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human YY1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PML polyclonal antibody |
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| AMR09397G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PML polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal AntibodyP5 Rabbit Polyclonal Antibody |
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| ES4829-100ul | ELK Biotech | 100ul | EUR 124 |
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Description: WB, IHC, ELISA |
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Polyclonal AntibodyP5 Rabbit Polyclonal Antibody |
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| ES4829-50ul | ELK Biotech | 50ul | EUR 74 |
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Description: WB, IHC, ELISA |
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Polyclonal AntibodyP3 Rabbit Polyclonal Antibody |
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| ES6454-100ul | ELK Biotech | 100ul | EUR 124 |
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Description: WB, IHC, ELISA |
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Polyclonal AntibodyP3 Rabbit Polyclonal Antibody |
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| ES6454-50ul | ELK Biotech | 50ul | EUR 74 |
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Description: WB, IHC, ELISA |
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Polyclonal AntibodyP2 Rabbit Polyclonal Antibody |
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| ES10016-100ul | ELK Biotech | 100ul | EUR 124 |
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Description: WB, ELISA |
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Polyclonal AntibodyP2 Rabbit Polyclonal Antibody |
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| ES10016-50ul | ELK Biotech | 50ul | EUR 74 |
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Description: WB, ELISA |
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Polyclonal AntibodyP4 Rabbit Polyclonal Antibody |
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| ES10017-100ul | ELK Biotech | 100ul | EUR 124 |
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Description: WB, ELISA |
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Polyclonal AntibodyP4 Rabbit Polyclonal Antibody |
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| ES10017-50ul | ELK Biotech | 50ul | EUR 74 |
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Description: WB, ELISA |
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Polyclonal AntibodyP1 Rabbit Polyclonal Antibody |
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| ES11752-100ul | ELK Biotech | 100ul | EUR 124 |
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Description: WB, ELISA |
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Polyclonal AntibodyP1 Rabbit Polyclonal Antibody |
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| ES11752-50ul | ELK Biotech | 50ul | EUR 74 |
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Description: WB, ELISA |
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Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody |
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| MBS9465999-005mL | MyBiosource | 0.05mL | EUR 300 |
Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody |
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| MBS9465999-01mL | MyBiosource | 0.1mL | EUR 390 |
Rabbit Polyclonal Antibody PC4 Rabbit Polyclonal Antibody |
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| MBS9465999-5x01mL | MyBiosource | 5x0.1mL | EUR 1610 |
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody |
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| MBS9465163-005mL | MyBiosource | 0.05mL | EUR 300 |
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody |
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| MBS9465163-01mL | MyBiosource | 0.1mL | EUR 390 |
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody |
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| MBS9465163-5x01mL | MyBiosource | 5x0.1mL | EUR 1610 |
Polyclonal RARA polyclonal antibody |
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| APR00370G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RARA polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EzH2 polyclonal antibody |
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| APR00373G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EzH2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BRD2 polyclonal antibody |
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| APR00374G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BMI1 polyclonal antibody |
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| APR00376G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EZH2 polyclonal antibody |
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| APR00436G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal RARA polyclonal antibody |
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| APR00437G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RARA polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal EZH2 polyclonal antibody |
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| APR00439G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CHD4 polyclonal antibody |
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| APR00444G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BRD2 polyclonal antibody |
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| APR00447G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Hira polyclonal antibody |
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| APR00449G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Hira polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SPI1 polyclonal antibody |
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| APR00451G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SPI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BMI1 polyclonal antibody |
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| APR00457G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CHD4 polyclonal antibody |
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| APR00459G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Rtf1 polyclonal antibody |
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| APR11187G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Rtf1 polyclonal antibody |
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| APR11188G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal SOX4 polyclonal antibody |
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| APR13475G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SOX4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MYH11 polyclonal antibody |
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| APR00438G | Leading Biology | 0.1ml | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYH11 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal CIITA polyclonal antibody |
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| APR00442G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CIITA polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal HDAC1 polyclonal antibody |
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| APR00445G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Spt16 polyclonal antibody |
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| APR00446G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal BCL7C polyclonal antibody |
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| APR00452G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BCL7C polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal LRWD1 polyclonal antibody |
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| APR00455G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LRWD1 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal KDM4D polyclonal antibody |
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| APR00458G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KDM4D polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Spt16 polyclonal antibody |
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| APR00460G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| AMM06350G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| AMM06351G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PADI4 polyclonal antibody |
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| APR08916G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PADI4 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PPARG polyclonal antibody |
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| AMR09459G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal PPARG polyclonal antibody |
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| AMR09486G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Med26 polyclonal antibody |
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| APR12517G | Leading Biology | 0.05mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal Med26 polyclonal antibody |
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| APR12518G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| APR12525G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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Polyclonal MeCP2 polyclonal antibody |
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| APR12526G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications: |
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