May 28, 2023

Modeling the Effect of Amino Acids and Copper on Monoclonal Antibody

genxbio

Modeling the Impact of Amino Acids and Copper on Monoclonal Antibody Productiveness and Glycosylation: A Modular Strategy

  • In manufacturing monoclonal antibodies (mAbs), it’s essential to have the ability to predict how course of situations and dietary supplements have an effect on antibody productiveness and high quality attributes, particularly glycosylation. Supplemental inputs, corresponding to amino acids and hint metals within the media, have been reported to have an effect on cell metabolism and glycosylation; understanding and quantifying their results are important for efficient course of improvement.
  • Our goal is to current and validate, by way of a commercially related cell tradition course of, a method for capturing such results quantitatively and effectively in a mannequin. Whereas at present out there fashions can predict mAb manufacturing or glycosylation dynamics precisely beneath particular course of configurations, adapting them to new processes stays difficult, as a result of it includes modifying all the mannequin construction and sometimes requires some mechanistic understanding of how cells behave beneath new situations.
  • Right here, we current a modular modeling method for adapting an current mannequin for a fed-batch Chinese language hamster ovary (CHO) cell tradition course of with out structural modifications or mechanistic perception. As an alternative, we use information, obtained from designed experimental perturbations in media concentrations of asparagine, glutamate, and copper, to coach and validate a supplemental enter impact mannequin, which approximates residual course of dynamics when supplemental inputs deviate from the baseline, and which is used to “patch” the prevailing mannequin.
  • The mixed mannequin can be utilized for process-specific, model-based course of improvement to enhance productiveness and to fulfill product high quality targets extra effectively. Our mannequin was developed for a selected CHO cell line; nonetheless, the modeling methodology and evaluation are usually relevant to different cell traces and cell varieties. This text is protected by copyright. All rights reserved.

Alpha-bungarotoxin CF488a

9-00005
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
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  • Ask for price
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 201.6
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 440.4
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 459.6
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 500.4
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 559.2
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 559.2
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 627.6
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 519.6
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • Ask for price
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • Ask for price
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 376.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 501.6
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 412.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 537.6
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 589.2
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 750
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 337.2
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 166.8
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 592.8
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 414
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 513.6
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 414
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 414
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 300EU
EUR 414
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T 50U
EUR 176.4
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079 100ug
EUR 338.4
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF680R conjugate

00086 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF750 conjugate

00087 1mg
EUR 186
Description: Minimum order quantity: 1 unit of 1mg

HIV-1 tat recombinant antigen

00110-V-01mg 0,1 mg
EUR 321
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 tat recombinant antigen

00110-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-01mg 0,1 mg
EUR 321
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 nef recombinant antigen

00112-V-01mg 0,1 mg
EUR 321
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 nef recombinant antigen

00112-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 env gp41 recombinant antigen

00113-V-01mg 0,1 mg
EUR 321
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-1 env gp41 recombinant antigen

00113-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-01mg 0,1 mg
EUR 321
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-1000ug 1000 ug
EUR 1539
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HCV core recombinant antigen

00115-V-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen

00115-V-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen, Biotin conjugate

00115-V-B-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, Biotin conjugate

00115-V-B-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b

00116-V-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b

00116-V-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS3 recombinant antigen

00117-V-01mg 0,1 mg
EUR 321
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HCV NS3 recombinant antigen

00117-V-1000ug 1000 ug
EUR 1539
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HBV core recombinant antigen

00120-V-01mg 0,1 mg
EUR 321
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen

00120-V-1000ug 1000 ug
EUR 1539
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen, Delta

00121-V-01mg 0,1 mg
EUR 321
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV core recombinant antigen, Delta

00121-V-1000ug 1000 ug
EUR 1539
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV surface recombinant antigen HBsAg

00122-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg

00122-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg antigen

00123-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00123-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00124-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HBV surface recombinant antigen HBsAg antigen

00124-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HEV (Birma) ORF2 recombinant antigen

00131-V-01mg 0,1 mg
EUR 321
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00131-V-1000ug 1000 ug
EUR 1539
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00132-V-01mg 0,1 mg
EUR 321
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461.

HEV (Birma) ORF2 recombinant antigen

00132-V-1000ug 1000 ug
EUR 1539
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461.

AA 403-461 HEV Birma ORF2 Recombinant Antigen

00133-V-01mg 0,1 mg
EUR 321
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123.

AA 403-461 HEV Birma ORF2 Recombinant Antigen

00133-V-1000ug 1000 ug
EUR 1539
Description: HEV (Birma) ORF2 recombinant antigen a.a. 92 to a.a 123.

T, Pallidum recombinant antigen p15

00141-V-01mg 0,1 mg
EUR 321
Description: T. Pallidum recombinant antigen p15 (full length)

T, Pallidum recombinant antigen p15

00141-V-1000ug 1000 ug
EUR 1539
Description: T. Pallidum recombinant antigen p15 (full length)

T, Pallidum recombinant antigen p17

00142-V-01mg 0,1 mg
EUR 321
Description: T. Pallidum recombinant antigen p17 (full length)

T, Pallidum recombinant antigen p17

00142-V-1000ug 1000 ug
EUR 1539
Description: T. Pallidum recombinant antigen p17 (full length)

T, Pallidum recombinant antigen p45

00143-V-01mg 0,1 mg
EUR 321
Description: T. Pallidum recombinant antigen p45 (full length)

Biased anti-idiotype response in rabbits results in high-affinity monoclonal antibodies to biologics

Antibody formation to human(ized) therapeutic antibodies in people is extremely skewed towards anti-idiotype responses, in all probability as a result of the idiotype is the one ‘international’ a part of the antibody molecule. Right here, we analyzed antibody responses to F(ab’)2 fragments of a panel of 17 human(ized) therapeutic antibodies in rabbits. Homology between the rabbit germline and the human(ized) antibodies is average not just for the variable domains (each the complementarity-determining regions and the framework areas), but in addition for the fixed domains (66% or much less).
However, we noticed a extremely skewed anti-idiotype response in all circumstances, with as much as >90% of the antibodies directed towards the idiotype. These outcomes point out that the idiotype could also be inherently immunodominant. We used these biased responses to lift monoclonal rabbit anti-idiotype antibodies in opposition to secukinumab, ustekinumab, reslizumab, mepolizumab, palivizumab, and dupilumab and display the potential to develop delicate pharmacokinetic assays with these antibodies.

Choice and Characterization of YKL-40-Concentrating on Monoclonal Antibodies from Human Artificial Fab Phage Show Libraries

YKL-40, often known as chitinase-3-like 1 (CHI3L1), is a glycoprotein that’s expressed and secreted by varied cell varieties, together with cancers and macrophages. Resulting from its implications for and upregulation in quite a lot of illnesses, together with inflammatory situations, fibrotic issues, and tumor progress, YKL-40 has been thought of as a big therapeutic biomarker. Right here, we used a phage show to develop novel monoclonal antibodies (mAbs) focusing on human YKL-40 (hYKL-40).
Human artificial antibody phage show libraries had been panned in opposition to a recombinant hYKL-40 protein, yielding seven distinctive Fabs (Antigen-binding fragment), of which two Fabs (H1 and H2) had been non-aggregating and thermally steady (75.5 °C and 76.5 °C, respectively) and had excessive obvious affinities (OkD = 2.Three nM and 4.Zero nM, respectively).
Reformatting the Fabs into IgGs (Immunoglobulin Gs) elevated their obvious affinities (notably, for H1 and H2, OkD = 0.5 nM and 0.Three nM, respectively), presumably because of the results of avidity, with little change to their non-aggregation property. The six anti-hYKL-40 IgGs had been analyzed utilizing a trans-well migration assay in vitro, revealing that three clones (H1, H2, and H4) had been notably efficient in lowering cell migration from each A549 and H460 lung most cancers cell traces.
The three clones had been additional analyzed in an in vivo animal check that assessed their anti-cancer actions, demonstrating that the tumor space and the variety of tumor nodules had been considerably decreased within the lung tissues handled with H1 (IgG). Given its excessive affinity and fascinating properties, we count on that the H1 anti-hYKL-40 mAb can be an appropriate candidate for growing anti-cancer therapeutics.

Polyclonal BRD2 polyclonal antibody

APR00374G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal JMJD2c polyclonal antibody

APR00375G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JMJD2c polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal BMI1 polyclonal antibody

APR00376G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal SHH Polyclonal Antibody

APR00434G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SHH Polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal ETO polyclonal antibody

APR00435G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ETO polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal EZH2 polyclonal antibody

APR00436G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal RARA polyclonal antibody

APR00437G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RARA polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal MYH11 polyclonal antibody

APR00438G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MYH11 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal EZH2 polyclonal antibody

APR00439G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EZH2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal p53K372me1 polyclonal antibody

APR00440G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human p53K372me1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal CIITA polyclonal antibody

APR00442G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CIITA polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal p53 polyclonal antibody

APR00443G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human p53 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal CHD4 polyclonal antibody

APR00444G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal HDAC1 polyclonal antibody

APR00445G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Spt16 polyclonal antibody

APR00446G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal BRD2 polyclonal antibody

APR00447G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BRD2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal G9a polyclonal antibody

APR00448G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human G9a polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Hira polyclonal antibody

APR00449G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Hira polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal JMJD2c polyclonal antibody

APR00450G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JMJD2c polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal SPI1 polyclonal antibody

APR00451G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SPI1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal BCL7C polyclonal antibody

APR00452G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BCL7C polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal LRWD1 polyclonal antibody

APR00455G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LRWD1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal LYDG10 polyclonal antibody

APR00456G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LYDG10 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal BMI1 polyclonal antibody

APR00457G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BMI1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal KDM4D polyclonal antibody

APR00458G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human KDM4D polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal CHD4 polyclonal antibody

APR00459G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CHD4 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Spt16 polyclonal antibody

APR00460G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Spt16 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal FKBP51 polyclonal antibody

APR15992G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FKBP51 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal JARID2 polyclonal antibody

APR12450G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JARID2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Med26 polyclonal antibody

APR12517G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Med26 polyclonal antibody

APR12518G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Med26 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal MeCP2 polyclonal antibody

APR12525G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal MeCP2 polyclonal antibody

APR12526G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal MeCP2 polyclonal antibody

APR12527G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MeCP2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal SOX4 polyclonal antibody

APR13475G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SOX4 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal YY1 polyclonal antibody

APR14018G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human YY1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal PADI4 polyclonal antibody

APR08916G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PADI4 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Rtf1 polyclonal antibody

APR11187G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal Rtf1 polyclonal antibody

APR11188G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Rtf1 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal PIWIL2 polyclonal antibody

APR17846G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PIWIL2 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal AKR1B10 Polyclonal Antibody

APG01659G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AKR1B10 Polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal ZMYND8 polyclonal antibody

AMM08559G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ZMYND8 polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal PML polyclonal antibody

AMR09397G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PML polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal PPARG polyclonal antibody

AMR09459G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal PPARG polyclonal antibody

AMR09486G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPARG polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal AntibodyP1 Polyclonal Antibody

ES11752-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against P1. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP1 Polyclonal Antibody

ES11752-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against P1. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP2 Polyclonal Antibody

ES10016-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against P2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP2 Polyclonal Antibody

ES10016-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against P2 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP4 Polyclonal Antibody

ES10017-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against P4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP4 Polyclonal Antibody

ES10017-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against P4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Polyclonal AntibodyP5 Polyclonal Antibody

ES4829-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against P5 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Polyclonal AntibodyP5 Polyclonal Antibody

ES4829-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against P5 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Polyclonal AntibodyP3 Polyclonal Antibody

ES6454-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against P3 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Polyclonal AntibodyP3 Polyclonal Antibody

ES6454-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against P3 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Polyclonal sRANK Ligand Polyclonal Antibody

APR00294G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human sRANK Ligand Polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal FGF-1 Polyclonal Antibody

APR00423G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FGF-1 Polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal RFX-AP polyclonal antibody

APR00441G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RFX-AP polyclonal . This antibody is tested and proven to work in the following applications:

Polyclonal EIF2C1/2 polyclonal antibody

APR00453G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EIF2C1/2 polyclonal . This antibody is tested and proven to work in the following applications: